Title: Application of ion pair staining system in process of bacterial protein staining
Abstract:Objective To evaluate a new protein ion pair staining system(adopting two kinds of material with reverse charge)for bacterial protein staining,and to compare its difference with similar foreign commer...Objective To evaluate a new protein ion pair staining system(adopting two kinds of material with reverse charge)for bacterial protein staining,and to compare its difference with similar foreign commercial staining kit.Methods Bacterial proteome of strains of Staphylococcus aureus and Vibrio parahaemolyticus were extracted with thermo solution and quantified with the RC DC kit and diluted with 4-folds gradient,and then followed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis.The gels were treated with dye staining and re-silver staining by contrast ion-pair system and imported commercial staining kits.Results The new type of dyes resulted in a more clear and obvious lane than that of foreign commercial staining kit with a bacterial protein limitation as low as 0.16 μg.Compared to foreign commercial staining kit,the operation for ion pairs silver staining system was more simple and the background was clearer;the display of bands was quick and clear.With ion pairs silver staining,the gels could withstand a longer period staining.In the process of ion pairs staining,the dye is a sensitizing agent for silver staining.The results of re-silver staining after dyeing were similar to the direct silver staining,and the process of sensitizing could be omited.Conclusion The dye used ion pair staining system has good compatibility with silver staining and is more suitable for the requirement of bacterial proteomics.Read More
Publication Year: 2011
Publication Date: 2011-01-01
Language: en
Type: article
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