Title: A Comparative Study on Two Staining Methods of Nucleic Acid
Abstract:Objective: To compare the effect and their sensitivity of two different staining methods to DNA in the gels, and evaluate the possible application of SYBR GreenⅠin routine DNA staining as a substitute...Objective: To compare the effect and their sensitivity of two different staining methods to DNA in the gels, and evaluate the possible application of SYBR GreenⅠin routine DNA staining as a substitute for ethidium bromide(EB). Methods: The new nucleic acid staining SYBR GreenⅠand EB were used to stain the DNA in the gel, perfectively stain before electrophoresis and after electrophoresis. Results: There was no distinct difference between the two methods. Both SYBR GreenⅠand EB stain could detect as little as 10 ng per band of DNA. Conclusion: The effect and sensitivity of the two staining methods are no distinct difference, and SYBR GreenⅠand EB may be used in these staining methods. SYBR GreenⅠ, which is a new nucleic acid stain may substitute for EB as a general dyestuff to stain the DNA in the gel.Read More
Publication Year: 2006
Publication Date: 2006-01-01
Language: en
Type: article
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