Title: Simultaneous staining with three fluorescent dyes of minute plankters on an agarose gel filter
Abstract: Abstract A new method, employing an agarose gel filter and triple staining with fluorescent dyes, was developed for observation and enumeration of planktonic microorganisms (0.2–20 μm in size range) from a variety of niches in the marine ecosystem. Dansyl chloride was used to stain the cell-surface proteins, Calcofluor white was used to stain cellulose and chitin, and DAPI was used to stain DNA. The stained specimens also could be examined by transmission light microscopy.
Publication Year: 1989
Publication Date: 1989-11-01
Language: en
Type: article
Indexed In: ['crossref']
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Cited By Count: 5
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