Title: Investigation of the Relationship between Ankylosing Spondylitis and HLA-B27 in Hunan Province
Abstract: Objective To evaluate the diagnostic significance of HLA-B27 antigen detection in patients with skeptical ankylosing spondylitis(AS) in Hunan province. Methods Peripheral blood mononuclear cells(PBMC) were isolated from venous blood of the patients with skeptical AS by Ficoll-Hypaque density centrifugation, and the blood samples were anticoagulated by glass beads. The HLA-B27 typing was performed by micro-lymphocyte cytotoxic test with standard soft drop assay. Result Among 647 patients with skeptical AS,male and femal were481 and 166 respectively.The male to femal ratio was about 3:1,and the percentage of HLA-B27 position was 67.15% and 25.90% respectively.Clinical manifestation of ankylosing spondylitis usually begin in late adolescence. Conclusion HLA-B27 antigen detection is an effective method on AS diagnosis and differential diagnosis.
Publication Year: 2002
Publication Date: 2002-01-01
Language: en
Type: article
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