Title: Determination of the Genistein in the Gubaoxinshen Tablets by HPLC
Abstract:ObjectiveTo determine the content of the genistein in the Gubaoxinshen Tablets by HPLC.MethodsThe Zoobax C_(18) Column(4.6 mm×250 mm)was used,the methyl cyanide-water(V∶V=30∶70) mixture as the mobile ...ObjectiveTo determine the content of the genistein in the Gubaoxinshen Tablets by HPLC.MethodsThe Zoobax C_(18) Column(4.6 mm×250 mm)was used,the methyl cyanide-water(V∶V=30∶70) mixture as the mobile phase,with the detection at 270 nm,the flow rate was 1.0 ml/min and column temperature was 40℃.ResultsThe linear calibration curves were obtained in the concentration ranges of 0.001 752~0.019 272 μg.The regression equation was Y=4 866 020.692 X-20.276,r=(0.999 9).The average recovery was 98.89%,and RSD was 0.91%.ConclusionThis method was convenient,accurate and specified.Read More
Publication Year: 2006
Publication Date: 2006-01-01
Language: en
Type: article
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