Title: Molecular Analysis of Phytase Gene Cloned from Bacillus subtilis
Abstract:Phytases are enzymes capable of hydrolyzing phytic acid to less-phosphorylated myo-inositol derivates. Phytases are becoming essential supplement to animal feeds. Bacillus sp. produces a thermostable ...Phytases are enzymes capable of hydrolyzing phytic acid to less-phosphorylated myo-inositol derivates. Phytases are becoming essential supplement to animal feeds. Bacillus sp. produces a thermostable phytase which renders it suitable for commercial production. Two strains of Bacillus subtilis NCDC-070 and NCIM-2712 were selected for cloning and sequencing of phy gene. Published phy gene gene sequences were used to design specific primers for amplification. B. subtilis strains amplified 1059bp product through PCR. The phy gene from B. subtilis NCDC-070 and NCIM-2712 were cloned in InsT/A vector and sequenced. The 928bp sequence from Bacillus strains NCIM-2712 and 826bp from NCDC-070 were compared with phy gene reference sequence. Comparison revealed 6 nucleotide and 3 amino acids variations. The phy gene from B. subtilis NCIM-2712 exhibited 99% similarity with one from NCDC-070 at nucleotide level.Read More
Publication Year: 2011
Publication Date: 2011-01-01
Language: en
Type: article
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Cited By Count: 7
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