Title: Cloning of phytase gene from Bacillus licheniformis and its expression in Escherichia coli
Abstract:According to the published phytase gene sequence from Bacillus licheniformis,primers were designed to amplify phytase gene by PCR.The PCR product was cloned to pMD18-T and sequenced.The phytase gene w...According to the published phytase gene sequence from Bacillus licheniformis,primers were designed to amplify phytase gene by PCR.The PCR product was cloned to pMD18-T and sequenced.The phytase gene we cloned comprises 1?146 nucleotides and encodes a polypeptide of 381 amino acids.Compared with published phyL gene sequence,the phytase gene showed 92?% identity on nucleotides level and 93?% identity on amino acids level. It was also cloned to the expression vector pHBM625 and overexpressed in E.coli.The expressed product has normal bioactivity.Read More
Publication Year: 2004
Publication Date: 2004-01-01
Language: en
Type: article
Access and Citation
AI Researcher Chatbot
Get quick answers to your questions about the article from our AI researcher chatbot