Title: Clone and expression of Bacillus subtilis apr gene
Abstract:Bacillus subtilis protease apr gene was cloned by PCR technology,the homology of this gene and code protein were compared,which enzymology characteristic was analyzed.It showed that full-length of gen...Bacillus subtilis protease apr gene was cloned by PCR technology,the homology of this gene and code protein were compared,which enzymology characteristic was analyzed.It showed that full-length of gene sequence was 1 509 bp,503 amino acid being coded,and the consistency of sequencing results was 100%.Recombinant expression plasmid pET-22b(+)-apr was constructed and expressed in E.coli BL21 successfully,protein molecular weight was 55 ku,and the determined enzyme activity was 19 500 U·mL-1.The cloning and analysis of this gene could provide valuable gene material for small peptide preparing of soybean protein,which had great significance on deep studying of the molecular biologicalmechanism.Read More
Publication Year: 2010
Publication Date: 2010-01-01
Language: en
Type: article
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