Title: Cloning of Phytase phyC from Bacillus subtilis WHNB02 and its Sequence Analysis
Abstract:The phyC encoding phytase was amplified by polymerase chain reaction (PCR) from Bacillus subtilis WHNB02.The amplified fragment was cloned and sequenced.The results show that:the coding region is 1152...The phyC encoding phytase was amplified by polymerase chain reaction (PCR) from Bacillus subtilis WHNB02.The amplified fragment was cloned and sequenced.The results show that:the coding region is 1152bp in size,encodes a peptide of 383 amino acid residues,in which there is a signal peptide with 26 amino acids.Seen from the phylogenetic analysis,the seven phytases from different Bacillus strains were classified into two groups.The DNA and protein sequences of phytase from Bacillus subtilis WHNB02 were submitted to GenBank,and the Accession Numbers are AF220075 and AA043434.1,respectively.Read More
Publication Year: 2004
Publication Date: 2004-01-01
Language: en
Type: article
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