Title: Cloning and Sequence Analysis of β-1,3-1,4-Glucanase Gene
Abstract:Based on the sequence alignment of Bacillus amyloliquefaciens,two pairs of primers were designed and synthesized in this study.Using the total DNA of Bacillus amyloliquefaciens as template,the gene of...Based on the sequence alignment of Bacillus amyloliquefaciens,two pairs of primers were designed and synthesized in this study.Using the total DNA of Bacillus amyloliquefaciens as template,the gene of β-1,3-1,4-glucanase(bgl) was amplified by PCR,which has about 750 bp in length.The gene was cloned into pTG19-T Easy vector,and the recombinant plasmid was identified by PCR,restriction enzyme analysis and sequencing.The homology analysis revealed that the nucleotide sequences of the cloned β-1,3-1,4-glucanase gene similar to the B.amyloliquefacines(M15674),B.subtilis(D00518) and B.licheniformis(AY365256)were 99%,95% and 94%,respectively.Read More
Publication Year: 2011
Publication Date: 2011-01-01
Language: en
Type: article
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