Title: The establishment of rabbit models with intrahepatic,intra-muscular and subcutaneous VX_2 tumor:a comparison study
Abstract: Objective To find out the tumor-bearing rabbit model which is most suitable for percutaneous interventional therapy by comparing the models receiving intrahepatic,intra-muscular or subcutaneous inoculation of VX-2 tumor. Methods A total of 54 New Zealand rabbits were randomly and equally divided into three groups. Rabbits of group A(n = 18) received intra-hepatic inoculation of VX-2 tumor. Rabbits of group B(n = 18) received intra-muscular inoculation of VX-2 tumor and rabbits of group C(n = 18) were subcutaneously inoculated with VX-2 tumor. Multi-planar CT perfusion-scanning was performed on the 12th,16thand 20thday after inoculation to determine the tumor size. Every 6 rabbits from each group were sacrificed each time at the 12th,16thand 20thday after inoculation. The specimens were collected and sent for pathologic examination. The operation time for complete modeling,the tumor size and the modeling-related complications were compared among the three groups. Results The VX-2 tumor of group A grew most fast and VX-2 tumor of group C grew slowest. The difference among the three groups was statistically significant(P 0.05). On the 12thday after inoculation the tumor size of group A was already about one cm in diameter,while in group C and group B the tumor size became one cm in diameter on the 20thday and 16thday after inoculation respectively. The operation time needed for modeling for group A,Band C was(5.54 ± 0.51) min,(1.02 ± 0.20) min and(0.98 ± 0.21) min respectively. The operation time needed for modeling of group A was the longest. Ascites,intrahepatic metastases as well as tumor implantation of abdominal wall were found in group A,while no obvious untoward events affecting VX-2 tumor model were observed in both group B and C. Conclusion Compared to intrahepatic and subcutaneous inoculation VX-2 tumor model,the establishment of intra-muscular inoculation VX-2 tumor model of rabbit is more simple in manipulation with higher tumor-formation rate,besides the growth rate of the tumor is moderate. Therefore,intra-muscular inoculation VX-2 tumor model of rabbit is most suitable for percutaneous interventional study.
Publication Year: 2013
Publication Date: 2013-01-01
Language: en
Type: article
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