Title: Detection of Id1 mRNA expression in prostate cancer by real time RT-PCR
Abstract:Objective To investigate the mRNA expression of Id1(inhibitor of DNA binding or inhibitor of differentiation)in human prostate cancer and its clinical significance.Methods The quantity of Id1 mRNA rel...Objective To investigate the mRNA expression of Id1(inhibitor of DNA binding or inhibitor of differentiation)in human prostate cancer and its clinical significance.Methods The quantity of Id1 mRNA relative to b-actin was detected by SYBR GreenⅠ-based real time RT-PCR in prostatectomy or biopsy samples of human normal prostate,benign prostate hyperplasia(BPH)and prostate cancer sam- ples.The relationship between the quantity of Id1 mRNA and some clinieopathological parameters,such as Gleason grade,prostate specific antigen(PSA)and clinical stage,was further analyzed.Results In pros- tate cancer tissues,the mean quantity of Id1 mRNA relative to b-actin was obviously higher than that in BPH(P0.01).The expression of Id1 mRNA was positively correlated with Gleason grade in prostate cancer group(r=0.9995,P0.05).One to two years after therapeutic prostateetomy,the probability of infiltration and relapse was obviously higher in the patients whose Id1 mRNA expression were markedly up- regulated.Conclusion The expression of Id1 mRNA is significantly increased in prostate eaneer,and it is negatively correlated with the differentiation level of prostate cancer.The high expression of Id1 mRNA re- fleeted its relationship with the progression of prostate cancer on the level of transcription.Read More
Publication Year: 2007
Publication Date: 2007-01-01
Language: en
Type: article
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