Title: Expressions and clinical significances of nuclear factor-κB and hypoxia inducible factor-1α in prostate cancer
Abstract:Objective:To study expressions and clinical significances of nuclear factor-κB(NF-κB) and hypoxia inducible factor-1α(HIF-1α) in prostate cancer.Methods:RT-PCR and immunohistochemistry SP method were ...Objective:To study expressions and clinical significances of nuclear factor-κB(NF-κB) and hypoxia inducible factor-1α(HIF-1α) in prostate cancer.Methods:RT-PCR and immunohistochemistry SP method were used to detect NF-κB and HIF-1α mRNA and protein expressions in 62 cases of prostate cancer tissues and 17 cases of prostatic hyperplasia tissues.Results:(1)RT-PCR results showed that NF-κB and HIF-1α mRNA expression levels were relatively significantly higher in prostate cancer group than in prostatic hyperplasia group,with statistical differences(P0.05).Immunohistochemical results showed that positive expression rates of NF-κB and HIF-1α proteins in prostate cancer group were 62.90% and 66.13%,8.33% and 5.88%,significantly higher than those in prostatic hyperplasia group with statistically significant differences(P0.05).(2)Expressions of NF-κB and HIF-1α were correlated with Gleason grade and clinical stages of prostate cancer(P0.05),but not with age(P 0.05).(3)Expressions of NF-κB and HIF-1α were positively correlated(rs=0.368,P0.05).Conclusions:Overexpression of NF-κB and HIF-1α may be involved in the pathogenesis and progression of prostate cancer.Moreover,NF-κB and HIF-1α may be correlated with the invasion and metastasis of prostate cancer.Read More
Publication Year: 2013
Publication Date: 2013-01-01
Language: en
Type: article
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