Title: Effects of Carbon Monoxide on Function and NO Production of Peripheral Bloodderived Endothelial Progenitor Cells in Vitro
Abstract: Objective:To investigate the effects of carbon monoxide(CO)on the proliferation,migration,adhesion and nitric oxide(NO) production of endothelial progenitor cells(EPCs) from human peripheral blood in vitro.Method:Total mononuclear cells were isolated from human peripheral blood by ficoll density gradient centrifugation and cultured on fibronectin-coated six-well plates.After 7 days,EPCs were identified with dual fluorescence staining and flow cytometry.EPCs were divided into control group and intervention group at the seventh day,and 25 μM,50μM,100 μM,200 μM carbon monoxide releasing molecule-3(CORM-3) were added in the intervention group,respectively.The proliferative,migratory and adhesive abilities and NO production were observed after 24 h.Results:Compared with control group,the proliferative,migratory and adhesive activities of EPCs were enhanced by CORM-3,and the NO concentration in the culture supematant were higher than the control group(P 0.05).In addition,CORM-3 had the most obvious effect on EPCs at 100μM level.Conclusion:CO could improve the proliferation,migration,adhesion capacity and NO production of EPCs in vitro,which suggests that CO could possibly play an role in accelerating re-endothelialization and promoting angiogenesis by improving the function of EPCs.
Publication Year: 2014
Publication Date: 2014-01-01
Language: en
Type: article
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