Title: Effects of ultrasound-mediated microbubble on nitric oxide and nitrogen synthase of endothelial progenitor cells.
Abstract: Objective To explore the mechanism of ultrasound-mediated microbubbles on nitric oxide(NO)and nitrogen synthase(NOS) of endothelial progenitor cells.Methods Bone marrow-endothelial progenitor cells(EPCs)of Chinese minipigs were extracted,separated and cultured in vitro.The EPCs were divided into 3 groups:control group,ultrasound group and ultrasound-mediated microbubble group.After the EPCs suffered from intervene for 90 seconds,the NO concentration in the supernatant of EPCs and NOS relative activity were measured by nitrate reduction and fluorescent probe with spectroscopy respectively.Results Compared to the control group,NO concentration in the supernatant of EPCs and NOS relative activity of EPCs were enhanced in ultrasound-mediated microbubble group,but no change was found in ultrasound group.Conclusion 1 W/cm2 ultrasound-mediated microbubbles might enhance the effect of stem cells treatment for myocardial infarction through increasing NO generation and enhancing the NOS activity.
Publication Year: 2010
Publication Date: 2010-01-01
Language: en
Type: article
Access and Citation
AI Researcher Chatbot
Get quick answers to your questions about the article from our AI researcher chatbot