Title: The Effect of 3,4-Dihydroxyacetophenone on Endothelial Progenitor Cells
Abstract: Objective:To investigate the effect of 3,4-Dihydroxyacetophenone on endothelial progenitor cells.Methods:EPCs were isolated from human umbilical cord blood.Cytochemical analysis was conducted after culture for 7 days.EPCs were characterized as that DiI-acLDL/FITC-UEA-I doublepositive cells were detected by laser confocalmicroscopy.These cells were Separated into 4 groups randomly:①control group:EPCs were suspended in endothelial cell basal medium(M199)supplemented with 20% fetal calf serum;②three groups with different concentration of 3,4-dihydroxyacetophenone:EPCs were suspended in M199 supplemented with10-4,10-5,10-6mol/L 3,4-Dihydroxyacetophenone,respectively.All groups were intervented for6,12,24,48 and 72hours,To compare the number of CFU,adhesion,migration and proliferation capability of EPCs.Results:10-4mol/L 3,4-Dihydroxyacetophenone markedly increase number of CFU,migration,adhesion and proliferation capability of EPCs with time accrescence,and it display time dependence.These parameters reach the peak at 72 hours.Conclusion:3,4-dihydroxyacetophenone may promote the repair of the damaged blood vessel endothelium and vasculogenesis through effecting the quantity,migration adhesion and proliferation capability of EPCs.
Publication Year: 2009
Publication Date: 2009-01-01
Language: en
Type: article
Access and Citation
AI Researcher Chatbot
Get quick answers to your questions about the article from our AI researcher chatbot