Title: The effect of As_2O_3 combined with INF on human epidermal carcinoma cells A431 in vitro
Abstract:Objective To investigate the inhibition effect of arsenic trioxide combined with interferon on the growth of epidermal carcinoma cells at different concentrations and different time in order to select...Objective To investigate the inhibition effect of arsenic trioxide combined with interferon on the growth of epidermal carcinoma cells at different concentrations and different time in order to select the optimized action time and dose of the combination of the two drugs.Methods Through cell culture with human epidermal carcinoma cells A431 in vitro,the inhibition effects of arsenic trioxide combined with INF at different concentrations on proliferation of A431 cells were detected by MTT.The apoptosis rate and the changes of cell cycle in human A431 cells were detected by FCM.Results The results of MTT showed that arsenic trioxide and INF respectively inhibited the proliferation of A431 cells in a dose-dependent and time-dependent manner.The combination of arsenic trioxide with INF inhibited the growth of A431 cells,and the inhibitory rate of the combination application was significantly higher than that of arsenic trioxide only,there was a significant difference between arsenic trioxide and INF(P0.01).There was a synergistic effect of arsenic trioxide and INF combination application.The synergistic effects of arsenic trioxide and INF on inducing apoptosis of A431 cells was more obvious,as compared with that of using simple arsenic trioxide or INF.Conclusion The arsenic trioxide and INF has inhibition effect on the growth of A431 cells in vitro respectively in a dose-dependent and time-dependent manner.The arsenic trioxide and INF can result in the arrearage of S phase of cell growth,and can increase the ration of S phase,and there is a synergistic effect of arsenic trioxide and INF combination application.The induction effect of arsenic trioxide on apoptosis of A431cells is quite obvious,nut INF can enhance the induction effect of arsenic trioxide on apoptosis of A431cells.Read More
Publication Year: 2011
Publication Date: 2011-01-01
Language: en
Type: article
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