Title: Effect of arsenic trioxide on proliferation and PPO expression in human melanoma cell line A375
Abstract:Objective To study the effect of arsenic trioxide on proliferation,apoptosis,cell cycles and phosphorylation oncogene(PPO) expression in the human melanoma cell line A375.Methods The human melanoma ce...Objective To study the effect of arsenic trioxide on proliferation,apoptosis,cell cycles and phosphorylation oncogene(PPO) expression in the human melanoma cell line A375.Methods The human melanoma cell line A375 was cultured.MTT assay was used to detect the effect of arsenic trioxide on proliferation, apoptosis and cell cycles of A375 cells at different concentrations.The effects of arsenic trioxide on cell cycle and apoptotic rates were assessed by flow cytometry.The morphological changes of cells were determined by the reverse microscopy after the treatment with arsenic trioxide.The influence of arsenic trioxide on the expressions of PPO in the A375 cells were detected with immunohistochemical method.Results It was confirmed by MTT assay that within the concentration interval of 1~10 μmol/L,as the concentration and exposure time increased,the inhibition ratio of arsenic trioxide on the A375 cells gradually increased.The inhibitory effects of arsenic trioxide on the proliferation of the A375 cells showed a time-and concentration-dependent manner.The growth of the A375 cells were arrested at S stage after treatment with arsenic trioxide with flow cytometry.It could be seen by the reverse microscopy that the typical morphological change of apoptosis occurred in the A375 cells.PPO protein was expressed in the intracytoplasm of the A375 cells.The expression of PPO protein diminished in arsenic trioxide-treated cells compared with the controls.There was difference between the control and the experiment groups(P0.05).Conclusions Arsenic trioxide significantly inhibits the proliferation of the A375 cells and there is a positive correlation between the concentration and effectiveness of the drug.The mechanism of action might be related to the apoptosis resulting from inhibition of PPO expression in the A375 cells.Read More
Publication Year: 2011
Publication Date: 2011-01-01
Language: en
Type: article
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