Title: Effect of Aspartate on Colonic Morphology and Barrier Function in Piglets with Lipopolysaccharide Challenge
Abstract:The study was conducted to evaluate the effects of aspartate(ASP) supplementation on colonic morphology and mucosal barrier function in piglets with lipopolysaccharide(LPS) challenge. Twenty-four pigl...The study was conducted to evaluate the effects of aspartate(ASP) supplementation on colonic morphology and mucosal barrier function in piglets with lipopolysaccharide(LPS) challenge. Twenty-four piglets were randomly assigned into 4 groups: control; LPS; 0.5% ASP; 1.0% ASP. The trial period was 19 days. On day 19, the piglets in the LPS group, 0.5% ASP group and 1.0% ASP group were injected with 100 μg/kg BW LPS, and the piglets in the control group were injected with the same dose of sterile saline. After 24 h saline or LPS injection, all pigs were sacrificed to obtain colonic samples for analysis. The results showed that: the colonic morphology from piglets in the control group was intact. Colonic epithelium falled off in LPS group; Colonic epithelium in ASP group falled off to less degree. Compared with the LPS group, colonic crypt depth of 0.5%ASP was significantly increased( P0.05). 0.5% or1.0% ASP alleviated the elevation of goblet cell number induced by LPS challenge( P 0.05). 0.5% ASP tended to increase the density of colon neutrophils( P 0.10). 0.5% or 1.0% ASP alleviated the decrease of DAO activity induced by LPS challenge(P 0.05). Theses results indicate that ASP supplementation can prevent injury of colonic morphology and barrier function induced by LPS challenge.Read More
Publication Year: 2015
Publication Date: 2015-01-01
Language: en
Type: article
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