Title: Alleviatory Effects of L-Arg on Intestinal Mucosal Barrier Damage of Weanling Piglets Induced by Lipopolysaccharide Challenge
Abstract:An experiment was conducted to study the effects of L-Arg on intestinal mucosal barrier damage induced by lipopolysaccharide (LPS) challenge in weanling piglets. Using single factorial experiment desi...An experiment was conducted to study the effects of L-Arg on intestinal mucosal barrier damage induced by lipopolysaccharide (LPS) challenge in weanling piglets. Using single factorial experiment design,eighteen 21-day-old Duroc×Landrace×Yorkshine crossed-bred piglets with (7.52±0.92) kg were randomly allotted to 3 groups (6 replicates per group and 1 piglet per replicate) including basal diet (control group),basal diet+LPS (LPS group) and basal diet+LPS+0.5% Arg (0.5% Arg group). The experiment lasted for 18 d. On day 18 of the experiment,piglets in LPS and 0.5% Arg groups were injected intraperitoneally with 100 μg/kg BW LPS,whereas piglets in control group were injected with an equivalent amount of physiological saline. Blood samples were collected 4 h after LPS challenge. Subsequently,the piglets were slaughtered to collect intestinal mucosa for analysis. The results showed as follows: 1) compared with control group,LPS challenge significantly decreased diamino oxidase (DAO) activity of duodenum mucosa (P0.05),and 0.5% Arg significantly increased DAO activity of duodenum and ileum mucosa compared with LPS group (P0.05); 2) compared with control group,LPS challenge had no significant effect on plasma level of D-lactate (P0.05),whereas 0.5% Arg significantly decreased plasma level of D-lactate compared with LPS group (P0.05); 3) compared with control group,LPS significantly increased endothelin-1 (ET-1) content of duodenum mucosa,and 0.5% Arg significantly alleviated the LPS-induced increase of ET-1 content in duodenum mucosa compared with LPS group (P0.05); 4) compared with control group,LPS challenge significantly increased the malondialdehyde (MDA) content of ileum mucosa,the GSH content of jejunum mucosa and GSH/GSSG of jejunum mucosa (P0.05),and 0.5% Arg significantly alleviated the increases of MDA in ileum mucosa and GSH in jejunum mucosa compared with LPS group. These results indicated that Arg has beneficial effects on alleviating intestinal mucosal barrier damage induced by LPS challenge by the protective effects of Arg associated with inhibiting ET-1 production and decreasing intestinal oxidative level.Read More
Publication Year: 2010
Publication Date: 2010-01-01
Language: en
Type: article
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