Title: Effect of berberine and yohimbine on impaired enterocyte proliferation and intestinal injury in LPS-challenged mice
Abstract:AIM:To evaluate the effects of oral berberine (Ber) and yohimbine (Y) in preventing intestinal damage and impaired enterocyte proliferation caused by lipopolysaccharide (LPS) in mice. METHODS:Male BAL...AIM:To evaluate the effects of oral berberine (Ber) and yohimbine (Y) in preventing intestinal damage and impaired enterocyte proliferation caused by lipopolysaccharide (LPS) in mice. METHODS:Male BALB/c mice were randomly divided into 8 groups:control,LPS,Ber + LPS,Ber + Y + LPS,Y + LPS,Ber,Ber + Y and Y. The mice were administered intragastrically with distilled water (0. 1 mL/10 g),Ber (50 mg/kg),Ber (50 mg/kg) in combination with Y (2 mg/kg) or Y (2 mg/kg) once a day for 3 days. One hour after intragastrical treatment on the third day,LPS (18 mg/kg) or normal saline was injected intraperitoneally. Twenty hours after LPS administration,the histological changes of the intestine were observed,and injury score was assessed. The mucosal weight,villus height and the content of diamine oxidase (DAO) in the ileum were also measured. Furthermore,the proliferation of enterocyte was identified by immunohistochemical staining for proliferating cell nuclear antigen (PCNA). RESULTS:Compared to the control mice,the mice challenged with LPS resulted in intestinal injury,including significantly increased injury score,decreased gut mucosa weight,villus height and the DAO contents in ileum. Furthermore,enterocyte proliferation was inhibited significantly 12 h after LPS challenge. Pretreatment with Ber or Ber + Y significantly inhibited the intestinal injury,and attenuated the impairment of enterocyte proliferation induced by LPS. However,no significant difference in the above parameters between Ber + LPS group and Ber + Y + LPS group was observed. Treatment with Y only did not prevent LPS-induced intestinal injury. CONCLUSION:Pretreatment with berberine remarkably reduces LPS-induced intestinal injury and the impairment of enterocyte proliferation in an alpha 2 adrenoceptor-independent manner.Read More
Publication Year: 2010
Publication Date: 2010-01-01
Language: en
Type: article
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Cited By Count: 1
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