Publication Information

{'id': 'https://openalex.org/W2368748569', 'doi': None, 'title': 'Cloning and Expression of β-1,3-1,4-glucanase Gene from Bacillus licheniformis', 'display_name': 'Cloning and Expression of β-1,3-1,4-glucanase Gene from Bacillus licheniformis', 'publication_year': 2004, 'publication_date': '2004-01-01', 'ids': {'openalex': 'https://openalex.org/W2368748569', 'mag': '2368748569'}, 'language': 'en', 'primary_location': {'is_oa': False, 'landing_page_url': 'https://en.cnki.com.cn/Article_en/CJFDTOTAL-NYSB200404018.htm', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S2915010491', 'display_name': 'Journal of Pharmaceutical and Biomedical Sciences', 'issn_l': '2230-7885', 'issn': ['2230-7885'], 'is_oa': False, 'is_in_doaj': False, 'is_core': True, 'host_organization': 'https://openalex.org/P4324004145', 'host_organization_name': 'Lawarence Press', 'host_organization_lineage': ['https://openalex.org/P4324004145'], 'host_organization_lineage_names': ['Lawarence Press'], 'type': 'journal'}, 'license': None, 'license_id': None, 'version': None, 'is_accepted': False, 'is_published': False}, 'type': 'article', 'type_crossref': 'journal-article', 'indexed_in': [], 'open_access': {'is_oa': False, 'oa_status': 'closed', 'oa_url': None, 'any_repository_has_fulltext': False}, 'authorships': [{'author_position': 'first', 'author': {'id': 'https://openalex.org/A5102685071', 'display_name': 'Xu Zi', 'orcid': None}, 'institutions': [], 'countries': [], 'is_corresponding': True, 'raw_author_name': 'Xu Zi', 'raw_affiliation_strings': ['-Rong**    DU Wen-Li    LI Wei-Feng    SUN Jian-Yi'], 'affiliations': [{'raw_affiliation_string': '-Rong**    DU Wen-Li    LI Wei-Feng    SUN Jian-Yi', 'institution_ids': []}]}], 'institution_assertions': [], 'countries_distinct_count': 0, 'institutions_distinct_count': 0, 'corresponding_author_ids': ['https://openalex.org/A5102685071'], 'corresponding_institution_ids': [], 'apc_list': None, 'apc_paid': None, 'fwci': 0.0, 'has_fulltext': False, 'cited_by_count': 2, 'citation_normalized_percentile': {'value': 0.425151, 'is_in_top_1_percent': False, 'is_in_top_10_percent': False}, 'cited_by_percentile_year': {'min': 68, 'max': 71}, 'biblio': {'volume': None, 'issue': None, 'first_page': None, 'last_page': None}, 'is_retracted': False, 'is_paratext': False, 'primary_topic': {'id': 'https://openalex.org/T10171', 'display_name': 'Biofuel production and bioconversion', 'score': 0.9916, 'subfield': {'id': 'https://openalex.org/subfields/2204', 'display_name': 'Biomedical Engineering'}, 'field': {'id': 'https://openalex.org/fields/22', 'display_name': 'Engineering'}, 'domain': {'id': 'https://openalex.org/domains/3', 'display_name': 'Physical Sciences'}}, 'topics': [{'id': 'https://openalex.org/T10171', 'display_name': 'Biofuel production and bioconversion', 'score': 0.9916, 'subfield': {'id': 'https://openalex.org/subfields/2204', 'display_name': 'Biomedical Engineering'}, 'field': {'id': 'https://openalex.org/fields/22', 'display_name': 'Engineering'}, 'domain': {'id': 'https://openalex.org/domains/3', 'display_name': 'Physical Sciences'}}, {'id': 'https://openalex.org/T11617', 'display_name': 'Enzyme Production and Characterization', 'score': 0.9902, 'subfield': {'id': 'https://openalex.org/subfields/1305', 'display_name': 'Biotechnology'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}, {'id': 'https://openalex.org/T10932', 'display_name': 'Microbial Metabolic Engineering and Bioproduction', 'score': 0.9366, 'subfield': {'id': 'https://openalex.org/subfields/1312', 'display_name': 'Molecular Biology'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}], 'keywords': [{'id': 'https://openalex.org/keywords/bacillus-licheniformis', 'display_name': 'Bacillus licheniformis', 'score': 0.8754703}, {'id': 'https://openalex.org/keywords/glucanase', 'display_name': 'Glucanase', 'score': 0.5358891}, {'id': 'https://openalex.org/keywords/cloning', 'display_name': 'Cloning (programming)', 'score': 0.44059125}], 'concepts': [{'id': 'https://openalex.org/C2776527165', 'wikidata': 'https://www.wikidata.org/wiki/Q2878356', 'display_name': 'Bacillus licheniformis', 'level': 4, 'score': 0.8754703}, {'id': 'https://openalex.org/C547475151', 'wikidata': 'https://www.wikidata.org/wiki/Q25419', 'display_name': 'Escherichia coli', 'level': 3, 'score': 0.7055095}, {'id': 'https://openalex.org/C86803240', 'wikidata': 'https://www.wikidata.org/wiki/Q420', 'display_name': 'Biology', 'level': 0, 'score': 0.6955362}, {'id': 'https://openalex.org/C104317684', 'wikidata': 'https://www.wikidata.org/wiki/Q7187', 'display_name': 'Gene', 'level': 2, 'score': 0.62054145}, {'id': 'https://openalex.org/C22744801', 'wikidata': 'https://www.wikidata.org/wiki/Q172778', 'display_name': 'Plasmid', 'level': 3, 'score': 0.6084207}, {'id': 'https://openalex.org/C153911025', 'wikidata': 'https://www.wikidata.org/wiki/Q7202', 'display_name': 'Molecular biology', 'level': 1, 'score': 0.6073221}, {'id': 'https://openalex.org/C2779530776', 'wikidata': 'https://www.wikidata.org/wiki/Q5572295', 'display_name': 'Glucanase', 'level': 3, 'score': 0.5358891}, {'id': 'https://openalex.org/C40767141', 'wikidata': 'https://www.wikidata.org/wiki/Q285697', 'display_name': 'Recombinant DNA', 'level': 3, 'score': 0.5231748}, {'id': 'https://openalex.org/C79029880', 'wikidata': 'https://www.wikidata.org/wiki/Q901755', 'display_name': 'GenBank', 'level': 3, 'score': 0.52141285}, {'id': 'https://openalex.org/C19924922', 'wikidata': 'https://www.wikidata.org/wiki/Q408351', 'display_name': 'Molecular cloning', 'level': 4, 'score': 0.4993391}, {'id': 'https://openalex.org/C84148353', 'wikidata': 'https://www.wikidata.org/wiki/Q863908', 'display_name': 'Nucleic acid sequence', 'level': 3, 'score': 0.45675382}, {'id': 'https://openalex.org/C150194340', 'wikidata': 'https://www.wikidata.org/wiki/Q26972', 'display_name': 'Gene expression', 'level': 3, 'score': 0.44806588}, {'id': 'https://openalex.org/C121050878', 'wikidata': 'https://www.wikidata.org/wiki/Q5135020', 'display_name': 'Cloning (programming)', 'level': 2, 'score': 0.44059125}, {'id': 'https://openalex.org/C55493867', 'wikidata': 'https://www.wikidata.org/wiki/Q7094', 'display_name': 'Biochemistry', 'level': 1, 'score': 0.26432255}, {'id': 'https://openalex.org/C2777272437', 'wikidata': 'https://www.wikidata.org/wiki/Q131238', 'display_name': 'Bacillus subtilis', 'level': 3, 'score': 0.20857239}, {'id': 'https://openalex.org/C54355233', 'wikidata': 'https://www.wikidata.org/wiki/Q7162', 'display_name': 'Genetics', 'level': 1, 'score': 0.19636679}, {'id': 'https://openalex.org/C523546767', 'wikidata': 'https://www.wikidata.org/wiki/Q10876', 'display_name': 'Bacteria', 'level': 2, 'score': 0.105323374}, {'id': 'https://openalex.org/C41008148', 'wikidata': 'https://www.wikidata.org/wiki/Q21198', 'display_name': 'Computer science', 'level': 0, 'score': 0.0}, {'id': 'https://openalex.org/C199360897', 'wikidata': 'https://www.wikidata.org/wiki/Q9143', 'display_name': 'Programming language', 'level': 1, 'score': 0.0}], 'mesh': [], 'locations_count': 1, 'locations': [{'is_oa': False, 'landing_page_url': 'https://en.cnki.com.cn/Article_en/CJFDTOTAL-NYSB200404018.htm', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S2915010491', 'display_name': 'Journal of Pharmaceutical and Biomedical Sciences', 'issn_l': '2230-7885', 'issn': ['2230-7885'], 'is_oa': False, 'is_in_doaj': False, 'is_core': True, 'host_organization': 'https://openalex.org/P4324004145', 'host_organization_name': 'Lawarence Press', 'host_organization_lineage': ['https://openalex.org/P4324004145'], 'host_organization_lineage_names': ['Lawarence Press'], 'type': 'journal'}, 'license': None, 'license_id': None, 'version': None, 'is_accepted': False, 'is_published': False}], 'best_oa_location': None, 'sustainable_development_goals': [], 'grants': [], 'datasets': [], 'versions': [], 'referenced_works_count': 0, 'referenced_works': [], 'related_works': ['https://openalex.org/W2472365816', 'https://openalex.org/W2393020191', 'https://openalex.org/W2386371604', 'https://openalex.org/W2383730821', 'https://openalex.org/W2383474745', 'https://openalex.org/W2380894555', 'https://openalex.org/W2376723605', 'https://openalex.org/W2370634359', 'https://openalex.org/W2364376266', 'https://openalex.org/W2356101125', 'https://openalex.org/W2356076067', 'https://openalex.org/W2347885442', 'https://openalex.org/W2112547049', 'https://openalex.org/W2094035349', 'https://openalex.org/W2069242438', 'https://openalex.org/W2019593322', 'https://openalex.org/W2017133351', 'https://openalex.org/W2005943302', 'https://openalex.org/W2004634964', 'https://openalex.org/W1965627587'], 'abstract_inverted_index': {'A': [0], 'gene': [1, 18, 52, 76, 86, 176], 'encodingβ-glucanase': [2], 'from': [3, 87], 'Bacillus': [4], 'licheniformis': [5], 'was': [6, 19, 91, 109, 128, 141, 145, 162], 'cloned': [7], 'and': [8, 28, 42, 63, 68, 73, 156, 167, 182], 'expressed': [9, 129, 139], 'in': [10, 66, 80, 101, 130], 'Escherichia': [11], 'coli..': [12], 'The': [13, 33, 47, 75, 85, 105, 116, 134, 154], 'whole': [14], 'length': [15], 'of': [16, 25, 39, 45, 50, 56, 61, 97, 118, 126, 137, 151], 'the': [17, 51, 159], '818': [20], 'bp,': [21], 'including': [22], 'an': [23], 'ORF': [24], '732': [26], 'bp': [27], 'encodeing': [29], '243': [30], 'amino': [31], 'acids.': [32], 'enzyme': [34, 135], 'showed': [35, 120], 'a': [36, 43, 172], 'molecular': [37], 'size': [38], '27.35': [40], 'kD': [41, 124], 'pI': [44], '8.31.': [46], 'nucleotide': [48], 'sequence': [49, 57], 'shared': [53], 'high': [54], 'degree': [55], 'similarity': [58], 'to': [59, 178], 'that': [60, 121, 150], 'B.pumilus': [62], 'B.licheniformis': [64], 'accessed': [65], 'GenBank,': [67], 'their': [69], 'similarities': [70], 'were': [71, 164], '99%': [72], '94%respectively.': [74], 'has': [77], 'been': [78], 'registered': [79], 'Genank(Accession': [81], 'number': [82], 'is': [83], 'AY225317).': [84], 'recombinant': [88, 106], 'cloning': [89], 'plasmid': [90, 99, 108], 'subcloned': [92], 'into': [93, 111], 'Bam': [94], 'HⅠand': [95], 'XhoⅠsite': [96], 'expression': [98, 107], 'pET-lic': [100], 'E.': [102, 112], 'coli': [103, 113], '.': [104], 'transformed': [110], 'strain': [114, 132, 140], 'BL21.': [115, 133], 'result': [117], 'SDS-PAGE': [119], 'about': [122, 146], '27': [123], 'protein': [125], 'theβ-1,3-1,4-glucanase': [127], 'E.coli': [131, 138], 'activity': [136, 161], '67.34': [142], 'U/mL,': [143], 'which': [144], '60-fold': [147], 'higher': [148], 'than': [149], 'original': [152], 'strain.': [153], 'pH': [155, 165], 'temperature': [157], 'at': [158], 'highest': [160], 'found': [163], '5~6': [166], '50': [168], '℃': [169], 'respectively.': [170], 'It’s': [171], 'good': [173], 'material': [174], 'for': [175], 'engineering': [177], 'construct': [179], 'highly': [180], 'active': [181], 'thermophilic': [183], 'enzymatic': [184], 'gene.': [185]}, 'cited_by_api_url': 'https://api.openalex.org/works?filter=cites:W2368748569', 'counts_by_year': [], 'updated_date': '2024-12-16T01:39:17.406631', 'created_date': '2016-06-24'}