Title: Panax Notoginseng Saponin Regulates c-Jun N-terminal Kinase to Improve Insulin Resistance of Rat Liver Tissue
Abstract: Objective To study the effect and mechanism of Panax Notoginseng Saponin(PNS) in attenuating insulin-resistance by exploiting insulin-resistant SD rats induced by high-fat and high-cholesterol diet.Methods 60 Sprague-Dawley(SD)male rats were randomly divided into three groups:control group(20 rats),model group(20 rats)and treatment group(20 rats).The rats in control group were fed with normal diet.By contrast,the rats in model group and the treatment group(20 rats) were intragastricly administrated with high fat and high cholesterol besides normal diet for 16 weeks to induce insulin resistance.The rats in treatment group were further intragastricly administrated with TPNS for 8 weeks.The rats in the two groups were intragastricly administrated with high fat and high cholestero until 24 weeks.MDA,GSH,GR and T-SOD in liver tissues were measured by chemical colorimetric analysis.And the c-Jun N-terminal kinase were determined by Western blotting.Results Triglyceride,total cholesterol,free fatty acid,insulin and insulin resistance index(IRI)of rats in the model group were significantly higher than in the control group(P0.05).The MDA of rats in model group was significant higher than in the control group(P0.05);the activities of GSH and GR of rats in model group were significantly lower than in the control group(P0.05).There was no significant difference(P0.05)in T-SOD among three groups.JNK phosphorylation increased in liver tissue of insulin-resistant model rats.After treatment of TPNS,hyperlipidemia and insulin resistance in rats in the treatment group were significantly attenuated(P0.05).PNS treatment reversed the process of phosphorylation of the above proteins.Conclusions PNS can attenuate hyperlipidemia of rats with insulin resistance and by increasing endogenous thiol antioxidant(enzyme) GSH,GR,level to protect liver tissue.At molecular level,PNS can attenuate insulin resistance by decreasing the activities of JNK in the insulin signaling pathway.
Publication Year: 2012
Publication Date: 2012-01-01
Language: en
Type: article
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