Title: QUERCTIN PROTECTIVE ACTION ON OXIDATIVE STRESS, SORBITOL, INSULIN RESISTANCE AND BETA-CELLS FUNCTION IN EXPERIMENTAL DIABETIC RATS
Abstract: Recent trends in controlling and treating diseases tend to prefer natural rather than synthetic ones. The present study was undertaken to investigate the protective effects and treating action of quercetin on insulin, insulin resistance and 6-cells functions also pancreatic sorbitol level and oxidative stress in diabetic rats. Thirty male albino rats (200–220 g) were randomly divided into three experimental groups: normal (group1) received equal volume of citrate buffer (pH 6.3) solution intraperitoneally (IP), diabetic (group 2) that induced by IP injection of STZ (30mg/kg body weight for three consecutive days), and quercetin diabetic (group 3) rats received quercetin (20mg/kg/day orally) for one week prior to STZ injection, and continued until the end of the study period (8 weeks). Blood samples were collected and the serum was separated for biochemical assays of glucose, insulin, nitric oxide (NO) and insulin resistance, 6-cells function were calculated. The pancreases of the sacrificed rats were excised and homogenized for biochemical assays of sorbitol, malondialdehyde (MDA) and antioxidant enzymes glutathione peroxidase (GSHPx), superoxide dismutase (SOD). Results: There were a significantly decreased in GSHPx, SOD, 6-cells function and insulin content in STZ diabetic group while, blood glucose concentrations, insulin resistance, MDA, sorbitol and NO significantly increased. The quercetin treated group showed a significant decrease in elevated blood glucose, insulin resistance, MDA, sorbitol, and NO. Furthermore, quercetin treatment significantly increased antioxidant enzyme’s activities, as well as insulin levels and 6-cell function. Conclusion , the findings of the present study concluded that quercetin treatment has beneficial effects on pancreatic tissues subjected to STZ-induced oxidative stress by directly quenching lipid peroxides and indirectly enhancing production of endogenous antioxidants. Also, it had 6-cells protective effect and prevent diabetic complication resulted in sorbitol formation.
Publication Year: 2011
Publication Date: 2011-01-01
Language: en
Type: article
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Cited By Count: 18
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