Title: Effects of Capillaris Decoction on Enhancing Insulin Sensitivity and Antagonizing Fatty Liver in Rats with Metabolic Syndrome
Abstract: Objective To investigate the prevention and treatment effects and mechanism of capillaris decoction on metabolic syndrome(Met S)-fatty liver(FL) in rats caused by feeding high-fat and high-sugar diet.Methods Excluding the blank control group eating standard chow and distilled water,the rats were fed with high-fat and high-sugar diet for 8 weeks,and then were randomly divided into fatty liver model group,silibinin group(150 mg·kg-1),and two groups of capillaris decoction(high dosage 5.0 g·kg-1,low dosage 2.5 g·kg-1).All the rats were still administered the same diet and given different drugs by intragastric administration for 4 weeks.After absolute diet 24 h,the levels of fasting insulin(Fins),fasting blood glucose(FBG),2-hours blood glucose(2h BG) after oral glucose tolerance test(OGTT),triglyceride(TG),total cholesterol(TC),low-density lipoprotein cholesterol(LDL-C),high-density lipoprotein cholesterol(HDL-C),free fatty acid(FFA),malonaldehyde(MDA) were determined;the activities of aspartate aminotransferase(AST),alanine aminotransferase(ALT),cholinesterase(ChE),and superoxide dismutase(SOD) were measured,as well as insulin sensitivity index(ISI),insulin resistance index(HOMA-IRI) and liver index were counted;livers were weighed and collected to be observed for liver histopathologic changes under light microscope.Results In MS-FL rats,the contents of FBG,OGTT-2h BG,TC,FFA,TG,LDL-C,MDA and Fins increased,the IRI and activities of AST,ALT,ChE,and liver index raised;ISI and activity of SOD were weakened,the contents of adiponectin was lowered in contrast to the normal control rats(P 0.05 or P 0.01);Livers histopathological change revealed adipose degeneration.The contents of FBG,OGTT-2h BG,TC,FFA,TG,LDL-C,MDA and Fins were lowered,the IRI and activities of AST,ALT,ChE,and liver index decreased,ISI and activity of SOD raised,the contents of adiponectin increased,these results were revealed by Silibinin 150 mg·kg-1 and the group of capillaris decoction 5.0 g·kg-1 compared with the MS-Ht rats(P 0.05 or P 0.01),and livers histopathological changes were ameliorated.Conclusions Capillaris decoction and silibinin are able to enhance the insulin sensitivity and modify hyperinsulinemia,antagonize disorder of lipid metabolism by down-regulating FFA,TG,TC,LDL-C and up-regulating HDL-C,adiponectin;augment the antioxidant capacity by increasing SOD and inhibiting form of MDA;relieve liver injury by interfering release of AST,ALT,ChE,the degrees of fatty liver lesion is depressed in MS-FL rats.The effects of capillaris decoction on anti-nonalcoholic fatty liver show dose-effect relationship.
Publication Year: 2012
Publication Date: 2012-01-01
Language: en
Type: article
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