Title: Determination of Quercetin from Viscum ovalifolium DC.by HPLC
Abstract: Objective To investigate the determination of quercetin from Viscum ovalifolium DC..Methods Hypersil C18(5 μm,4.6 mm×250 mm) column was chosen as analytic column with mobile phase consisted of methanol-0.5% phosphoric acid(43∶57),the flow rate was 1.0 mL/min,the detection wavelength was set at 372 nm and the column temperature was at 30℃.Results The liner of quercetin was 0.06 to 1.32 μg.The average recovery was 98.89% with RSD = 2.66%(n = 6).The content of quercetin from Viscum ovalifolium DC.was 0.28 mg/g.Conclusion This method is high sensitivity,good reproducibility,and can be used for determination of quercetin from Viscum ovalifolium DC.and with reference value on the control of Viscum ovalifolium DC..
Publication Year: 2012
Publication Date: 2012-01-01
Language: en
Type: article
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Cited By Count: 1
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