Title: Determination of total flavonol in viscum coloratura capsules by HPLC
Abstract: OBJECTIVE To develop a HPLC method for the determination of total flavonol in viscum coloratura capsules by HPLC.METHODS The chromatographic analysis was performed on Kromasil C18(4.6×250mm,5μm)column.The mobile phase consisted of methonal-0.4% phosphoric acid(55∶45).The flow rate was 1.0 mL·min-1.The detection wavelength was at 360nm.RESULTS Quercetin:A good linearity was obtained over the range of 4.067μg·mL-1~40.67μg·mL-1(r=0.9998).Kaempferol:A good linearity was obtained over the range of 0.871 μg·mL-1~8.71μg·mL-1(r=0.9998).Isorhamnetin:A good linearity was obtained over the range of 0.616μg·mL-1~6.16μg·mL-1 μg·mL-1(r=0.9996).CONCLUSION The method was simple,accurate,specific and would be used for the quality control of quercetin,kaempferol and isorhamnetin in crudesaponins of viscum coloratura.
Publication Year: 2007
Publication Date: 2007-01-01
Language: en
Type: article
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