Title: HPLC Determination of Quercetin in Polygonum flaccidum from Different Growing Areas
Abstract: Objective:To determine the quercetin content of Polygonum flaccidum from different growing areas.Method:The chromatographic column was Hypersil BDS C18(4.6 mm×250 mm,5 μm).The mobile phase was methanol-0.4%phosphoric acid(50∶50).The flow rate was 1 mL · min-1,the detection wavelength was at 365 nm.The column temperature was at room temperature.The sample volume was 20 μL.Result:The standard curve regression equation was A=73 185C-21.47(r=0.999 5).It showed a good linear relationship between 0.004-0.014 g · L-1,and average recovery was 99.29%,RSD 1.30%.Conclusion:The method is simple and reliable,could be used to control the quality of these medicinal materials.
Publication Year: 2012
Publication Date: 2012-01-01
Language: en
Type: article
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