Title: Content Determination of Quercetin in Zornia diphylla by HPLC
Abstract: OBJECTIVE:To establish a method for the content determination of quercetin in Zornia diphylla. METHODS:HPLC method was adopted. The determination was performed on Agilent TC-C18(250 mm×4.6 mm,5 μm) column with mobile phase consisted of methanol-0.1% aqueous phosphoric acid solution(55:45,V/V) at the flow rate of 1.0 mL·min-1. The detection wavelength was set at 360 nm and injection volume was 10 μL. RESULTS:The linear range of quercetin was 0.039~0.630 μg(r=0.999 9) with an average recovery of 100.40%(RSD=1.9%,n=6) . CONCLUSION:The method is accurate,rapid,simple and reproducible,for the quality control of Z. diphylla.
Publication Year: 2012
Publication Date: 2012-01-01
Language: en
Type: article
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