Title: Determination of chlorogenic acid in Kang 601 mixture by HPLC
Abstract: Aim To determine chlorogenic acid as the active ingredient of Flos Lonicerae in Kang 601 Mixture by HPLC.Methods The chromatographic column was Hypersil ODS(4.0 mm×125 nm,5 μm).The mobile phase was methanol-water-acetic acid(15∶ 85∶ 0.5).The detection wavelength was set at 327 nm.The temperature was 27℃ with the flow rate of 1.0 ml·min-1.Results The linear range of chlorogenic acid was 0.2~2 μg(r=0.999 8).The average recovery was 98.3%,and RSD was 0.79%(n=6).Conclusion The method is simple,accurate and reproducible for quality control of Kang 601 Mixture.
Publication Year: 2011
Publication Date: 2011-01-01
Language: en
Type: article
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Cited By Count: 1
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