Title: Determination of chlorogenic acid in Sanhuajiedu yin by HPLC
Abstract: Aim To assay the content of chlorogenic acid in Sanhua jiedu yin by HPLC.Methods With gradient elution,Kromasil C18(4.6 mm × 250 mm)was used as chromatograthic column with the acetonitrile 0.5% phosphoric acid as the mobile phase.The column temperature was 35 ℃,the ultraviolet detection wavelength for chlorogenic acid was 326 nm,and the flow rate was 1.0 ml·min-1.Results The progress of chlorogenic acid presented a good linearity with the peak square in the range from 0.162 3 to 0.814 9 μg,r=0.999 8(n=5).The average rate of recovery was 98.04%,and RSD was 0.97%(n=3).Conclusion This method is specific,reproducible and can be used as content control standards.
Publication Year: 2011
Publication Date: 2011-01-01
Language: en
Type: article
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