Title: Optimization of Enrichment and Purification Technology for Total Iridoid Glycosides from Jasminum officinale by Macroporous Resin
Abstract:Objective: To optimize enrichment and purification technology of total iridoid glycosides from Jasminum officinale by macroporous resin.Method: With oleuropein as index,its content was determined by H...Objective: To optimize enrichment and purification technology of total iridoid glycosides from Jasminum officinale by macroporous resin.Method: With oleuropein as index,its content was determined by HPLC.Types of macroporous resin were optimized by static adsorption-elution test,enrichment and purification technology was investigated by orthogonal test and single-factor tests.Result: XDA-16 macroporous resin showed better effect than others,optimum purification technology was as following: the concentration of sample liquid 0.08 g.mL-1,height-diameter ratio of 1∶ 3,absorption velocity 2 BV.h-1,dosage of sample liquid of 0.7 g.g-1,washed impurity with 8 BV water,then eluted wtih 4 BV 70% ethanol at 2 BV.h-1,collected eluent.Conclusion: This optimized technology was stable and feasible.XDA-16 macroporous resin had a good enrichment and purification effect on total iridoid glycosides from J.officinale.Read More
Publication Year: 2013
Publication Date: 2013-01-01
Language: en
Type: article
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