Title: Optimization of Enrichment and Purification Technology for Total Glycosides from Acanthopanax giraldii by Macroporous Resin
Abstract:Objective:To optimize technology conditions and technical parameters of enrichment and purification process for total glycosides from Acanthopanax giraldii by macroporous resin.Method:A RP-HPLC method...Objective:To optimize technology conditions and technical parameters of enrichment and purification process for total glycosides from Acanthopanax giraldii by macroporous resin.Method:A RP-HPLC method was adopted to determine the content of total glycosides in A.giraldii with elution ratio of syringin and oleanolic acid as indexes,adsorption characteristics and elution parameters of enrichment and purification technology were investigated from 10 kinds of macroporous resin.Result:X-5 was the best macroporous resin;Optimum purification technology was:the concentration of total glycosides extraction from A.giraldii 0.3 g ·mL-1,pH of liquid 10,eluted with 4 BV 80% ethanol.Conclusion:X-5 macroporous resin could be well used for enrichment and purification of total glycosides from A.giraldii.Read More
Publication Year: 2012
Publication Date: 2012-01-01
Language: en
Type: article
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