Title: The isolation and purification technology of four triterpenoids from Alismatis Rhizoma by macroporous resin
Abstract:Objective To study on the isolation and purification technology of four triterpenes from Alismatis Rhizoma by macroporous resin.Methods The optimization of the isolation was performed by static and dy...Objective To study on the isolation and purification technology of four triterpenes from Alismatis Rhizoma by macroporous resin.Methods The optimization of the isolation was performed by static and dynamic adsorption parameters with 24-acetyl alisol A,alisol B,23-acetyl alisol B and 11-deoxy-23-acetyl alisol B in Alismatis Rhizoma as indicators.Results D-4020 macroporous resin showed the best performance for simultaneous separation and purification of 4 triterpenoids.Optimal parameters were as follows: the crude drug concentration of 0.50 g/mL,resin and the(g:g) with 1:1,first eluting with distilled water,then 50% ethanol eluting for 5 BV,and 90% ethanol for 8 BV with elution flow rate of 2 BV/h.90% ethanol eluting to collect and dry to obtain the solid samples with four triterpenoids' content of 18.68%.Conclusion D-4020 macroporous resin in the process conditions may be suitable for the simultaneous purification of the triterpenoids.Read More
Publication Year: 2012
Publication Date: 2012-01-01
Language: en
Type: article
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Cited By Count: 1
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