Title: Isolation,Identification and Homology Analysis of Non-structural Protein Nsp2 Gene of High Pathogenic Porcine Reproductive and Respiratory Syndrome GD07b Strain
Abstract: In this research,samples of lung and lymph node of dead pigs with suspicious highly pathogenic porcine reproductive and respiratory syndrome(HPPRRS) were collected from a pig farm.The genome of PRRSV with Nsp2 segment deletion was detected from these samples by RT-PCR.The samples,which were determined to be PRRSV positive by RT-PCR,were infected to the Marc-145 cells and cultured for 72 to 96 h until the monolayer cells showed obvious CPE.The virus was harvested and named as HPPRRSV-GD07b.According to the PRRSV sequences published on GenBank,a pair of specific primers was designed and used for RT-PCR amplification gene Nsp2 of PRRSV GD07b isolate.The length of product was 200 bp,which was 90 bp shorter than that of the VR-2332 strain,a classic PRRSV.After purification,the amplification product was sequenced.The sequence was compared with those of Nsp2 gene of other 19 strains PRRSV.The homology of Nsp2 gene of this isolate and 4 classic strains like CH-1a was relatively low to be 60.5% to 81.6%,while the homology between this isolate and other 14 variant strain was 90.6% to 98.9%.
Publication Year: 2010
Publication Date: 2010-01-01
Language: en
Type: article
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