Title: Genetic Variations of Nsp2 Gene and ORF5 Gene of Porcine Reproductive and Respiratory Syndrome Virus Isolates
Abstract: To fully understand the knowledge of highly pathogenic porcine reproductive and respiratory syndrome(PRRSV) molecular epidemiology and genetic variation in Shandong area,a total of 14 PRRSV isolates were isolated in 2007~2009,and Nsp2 gene and ORF5 gene of these isolates were amplified by RT-PCR and sequenced.Comparing with JXA1 strain,Nsp2 gene shared 96.1%~99.8% of nucleotide homologies and 92.9%~100% of the deduced amino acid homologies,while the ORF5 gene shared 98.0%~99.8% of nucleotide homologies and 97.5%~99.5% of the deduced amino acid homologies.Sequence results showed that all the 14 PRRSV isolates had discontinuous 30 amino acids deletions in Nsp2;the GP5 protein encoded by ORF5 gene had a few mutation but no deletion.The phylogenetic tree revealed JQ strain isolated in 2007 had closer relationship with JXA1 strain.Among the 12 PRRSV strains isolated in 2009,two strains still locate in the same branch with the domestic strains isolated between 2006 and 2007,nine strains constitute an independent branch,and 1 strain(DLS-1) forms in a separate branch,indicating that those PRRSV isolates have no obvious geographical characteristics but have an annual feature in Shandong area.
Publication Year: 2011
Publication Date: 2011-01-01
Language: en
Type: article
Access and Citation
AI Researcher Chatbot
Get quick answers to your questions about the article from our AI researcher chatbot