Title: Cloning and sequence analysis of the Nsp2 gene of porcine reproductive and respiratory syndrome virus isolated in Guizhou area.
Abstract: To investigate the genetic diversity and molecular epidemiology of porcine reproductive and respiratory syndrome virus(PRRSV) in Guizhou area,the DNA fragments encoding partial Nsp2 of PRRSV isolates from Guizhou were amplified by RT-PCR and sequenced.All 12 PRRSV isolates were classified into the same clade together with the previously reported highly pathogenic PRRSV(HP-PRRSV).Eight of the isolates had 2 discontinuous deletions(aa 481 and aa 533 to aa 561) in Nsp2 protein that showed a typical structure as previously reported for HP-PRRSV.Other 4 isolates had further deletions,of which 3 isolates were found a deletion at aa 471 to aa 500 in Nsp2 protein,and one amino acid deletion at the position of aa 394 in another isolate.The emergence of these new isolates indicated that the HP-PRRSV may evolve towards to a new trend.
Publication Year: 2010
Publication Date: 2010-01-01
Language: en
Type: article
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