Title: Adjuvant effect of chicken IL-18 on the immune responses to DNA vaccine against avian Pasteurella multocida
Abstract:Five-week-old chickens were inoculated intramuscularly with pcDNA3/cIL-18,pcDNA3/cIL-18 and pcDNA3/ompH,co-expressing plasmid pcDNA3/ompH-cIL-18 or pcDNA3/ompH respectively to elucidate the adjuvant e...Five-week-old chickens were inoculated intramuscularly with pcDNA3/cIL-18,pcDNA3/cIL-18 and pcDNA3/ompH,co-expressing plasmid pcDNA3/ompH-cIL-18 or pcDNA3/ompH respectively to elucidate the adjuvant effect of chicken IL-18 on the DNA vaccines expressing the outer membrane protein of avian Pasteurella multocida C48-1.After first immunization,peripheral blood lymphocytes were detected by MTT and the sera were collected to examine antibodies of chickens each week by ELISA.The chickens were challenged with 10 LD50 avian P.multocida C48-1 on week 2 post the booster.The results showed that chicken IL-18 was able to improve obviously the immunogenicity of the DNA vaccine,and to enhance the humoral immunity level and cellular immunity level of the vaccinated chickens.The adjuvant effect of IL-18 was the strongest when it was co-expressed with H gene in eukaryotic expression vector pcDNA3.Therefore,chickens vaccinated with recombinant DNA vaccine pcDNA3/ompH-cIL8 were protected effectively against challenge with avian P.multocida C48-1.Read More
Publication Year: 2007
Publication Date: 2007-01-01
Language: en
Type: article
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Cited By Count: 1
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