Title: Immune efficacy of the attenuated Salmonella typhimurium harbouring DNA vaccine against avian influenza virus
Abstract:The hemagglutinin protein(HA) gene of avian influenza virus was amplified from pVAX1-HA by PCR and sub-cloned into pcDNA3.1(+) vector.HA-CpG gene was also cloned into the pcDNA3.1(+).The two recombina...The hemagglutinin protein(HA) gene of avian influenza virus was amplified from pVAX1-HA by PCR and sub-cloned into pcDNA3.1(+) vector.HA-CpG gene was also cloned into the pcDNA3.1(+).The two recombinant plasmids were transfected respectively into COS-7 cells,and identified for the transient expression of HA gene by indirect immunofluorescent assay.Finally,the two recombinant plasmids were transformed into the attenuated Salmonella typhimurium SL7207,and the recombinants were designated as SL7207(pcDNA3.1-HA) and SL7207(pcDNA3.1-HA-CpG),respectively.One-day-old commercial ISA brown chickens were immunized with one of the 2 recombinant bacteria at 5×109 CFU,respectively and boosted with the same dose two weeks later.No significant difference was observed in body weight among those groups.Intestinal mucosal immune response was observed in chickens immunized with one of the 2 recombinant bacteria on day 35,and their levels were significantly higher than those of the negative and positive controls.Compared with the negative control group,protective immunity induced by SL7207(pcDNA3.1-HA-CpG) provided significant protection,but SL7207(pcDNA3.1-HA) did not,indicating that the combination of CpG can enhance the immune response of the DNA vaccine deli-vered by the attenuated Salmonella.Read More
Publication Year: 2007
Publication Date: 2007-01-01
Language: en
Type: article
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