Title: Immunization of newcastle disease virus F gene DNA vaccine with chicken IL-18 gene eukaryotic expression plasmid.
Abstract:To study the effect of immunization of newcastle disease virus(NDV) F DNA vaccine with chicken IL-18 gene eukaryotic expression plasmid(pcDNA-chIL-18),F gene of NDV strain F48E9 was amplified by RT-PC...To study the effect of immunization of newcastle disease virus(NDV) F DNA vaccine with chicken IL-18 gene eukaryotic expression plasmid(pcDNA-chIL-18),F gene of NDV strain F48E9 was amplified by RT-PCR.The eukaryotic expression plasmid pcDNA-F(DNA vaccine) was constructed by inserting F gene into the pcDNA 3.1 vecter.11-day-old chickens were divided into 4 groups,10 chickens per group.14-day-old chickens were primarily immunized by injected intramuscularly and boosted immunized 14 days later after primary immunization,respectively with pcDNA-F,pcDNA-F combined pcDNA-chIL-18,ND attenuated vaccine and NS.On days 7,14,21 and 28 post-immunization,peripheral blood antibody response to NDV and T lymphocyte transformation ratio were detected by ELISA and LTT(MTT),respectively.Every chicken was challenged with 50LD50 NDV strain F48E9 at 29 day post immunization.The results showed that the pcDNA-F group obtained 3/10 protection,the pcDNA-F combined pcDNA-chIL-18 group obtained 5/10 protection.Their protection effect were less than that of ND conventional vaccine group(10/10).Antibody titers against NDV of the pcDNA-F combined pcDNA-chIL-18 group had little difference than that of the pcDNA-F group(P0.05).T lymphocyte transformation ratio of the pcDNA-F combined pcDNA-chIL-18 group was significantly higher than that of the pcDNA-F group(P0.05).It is concluded that pcDNA-F could produce some protection to the immunized chickens,and pcDNA-chIL18 could produce some immunoenhancing effects to the chickens immunized with pcDNA-F.Read More
Publication Year: 2009
Publication Date: 2009-01-01
Language: en
Type: article
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