Title: The immune efficacy of ompH-DNA vaccine against avian Pasteurella multocida in chickens
Abstract:To investigate the immune efficacy of DNA vaccine encoding out membrane protein H(OmpH) of avian Pasteurella multocida,the omph gene was cloned into the eukaryotic expression vector pcDNA3.1(+) to con...To investigate the immune efficacy of DNA vaccine encoding out membrane protein H(OmpH) of avian Pasteurella multocida,the omph gene was cloned into the eukaryotic expression vector pcDNA3.1(+) to construct the recombinant plasmids pOMPH.The immune responses to pOMPH were evaluated based on serum antibody titers,lymphocyte proliferation assay and titers of the cytokines IFN-γ.The protective efficacy following challenge with a virulent avian P.multocida strain was evaluated based on protection rate.The result showed that a significant increase in serum antibody levels was detected in chickens vaccinated with pOMPH and attenuated live vaccine(p0.01).The lymphocyte proliferation(SI values) and the levels of IFN-γ were higher in chickens immunized with pOMPH than in attenuated live vaccine.Furthermore,pOMPH vaccine provided partial protection to the vaccinated chickens(66.7%);however,the protective efficacy was inferior to that provided by attenuated live vaccine(73.3%).This finding might be useful for the development of avian P.multocida novel vaccines.Read More
Publication Year: 2012
Publication Date: 2012-01-01
Language: en
Type: article
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