Title: Content Determination of Baicalin in Fengganning Powder by HPLC
Abstract: [Objective] The research aimed to establish a HPLC method for the determination of baiealin in Fengganning powder.[Method] The determination was performed by HPLC using a CLC-ODS C18 column(150.0 mm×6.0 mm,5 μm),with mobile phase consisted of methanol/water(45∶55) at a flow rate of 0.5 ml/min.And the detection wavelength was 277 nm.The content of baicalin in sample solution was determined.[Result] The calibration curve for baicalin was linear in the concentration range of 2.48-12.79 mg/ml(r=0.999 8).The average recovery was 98.95%(RSD=0.71%,n=9).The average content of baicalin in 7 batches of samples was 6.02 mg/ml.[Conclusion] The determination of baicalin in Fengganning Powder by HPLC was simple,rapid and applicable for the quality control of Fengganning Powder.
Publication Year: 2010
Publication Date: 2010-01-01
Language: en
Type: article
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