Title: Effect of Butylphthalide on Apoptosis of SH-SY5Y Cells
Abstract:Objective To explore the effects of butylphthalide on the apoptosis of SH-SY5 Y cells induced by the 1-methyl-4-phenylpyridinium iodide(MPP+). Methods SH-SY5 Y cells were cultured with butylphthalide ...Objective To explore the effects of butylphthalide on the apoptosis of SH-SY5 Y cells induced by the 1-methyl-4-phenylpyridinium iodide(MPP+). Methods SH-SY5 Y cells were cultured with butylphthalide in the dosage of 1 μmol/L, 10 μmol/L and 20 μmol/L for2 hours, and with MPP+for 24 hours. The viability of cells was measured with trypan blue. The mitochondrial membrane potential was detected with Rhodamine 123. The content of P53 protein was detected with ELISA. The expression of P53 and cytochrome C(Cyt C) were detected with Western blotting. Results The viability of SH-SY5 Y cells increased with the dosage of butylphthalide(P0.05), while the mitochondrial membrane potential and the expression of P53 and Cyt C decreased(P0.05). Conclusion Butylphthalide can prevents the SH-SY5 Y cells from the injury induced by MPP+, which may associate with the inhibition of apoptosis through the expression of P53 and CytC.Read More
Publication Year: 2015
Publication Date: 2015-01-01
Language: en
Type: article
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