Title: Protective effects of paeoniflorin against hydrogen peroxide-induced cell injury in SH-SY5Y nerve cells
Abstract: Objective To investigate the neuroprotective effects of paeoniflorin(PF) against cell death induced by hydrogen peroxide(H2O2) in human neuroblastoma cells and its mechanisms.Methods H2O2was used to induce SH-SY5Y cell damages,and the cell survival rate was detected by CCK-8 assay;the cell morphologic changes were observed by inverted optical microscope;the apoptosis was tested using Hoechst 33258 staining;flow cytometer(FCM) and propidium iodide staining were used to analyze the apoptosis and cell cycle alteration;reactive oxygen species(ROS) production was determined by 2′,7′-dichlorodihydrofluorescein diacetate(DCFH-DA) fluorescence;lactic dehydrogenase(LDH) release was detected by reaction of diaphorase and INT;8-OHdG production was determined by enzyme-linked immunosorbent assay(ELISA);caspase-3 activity was determined by caspase-3 catalyzing the specific substrate.Results Compared with control group,after the treatment with H2O2(200 μmol/L) for 24 h,the viability and proliferation index of CH-SY5Y cells were significantly decreased(P 0.01,0.05),the apoptosis rate and content of 8-OHdG were increased(P 0.01),the LDH resease and ROS production were increased(P 0.01);the activity of caspase 3 was increased(P 0.01).Compared with H2O2injury group,PF(20—40 μmol/L) significantly ameliorated the changes in SH-SY5Y cells induced by H2O2in concentration-related manner(P 0.05).PF(10 μmol/L) did not significantly change the above indexes except the cell viability,ROS,and caspase-3 activity induced by H2O2(P 0.05).Conclusion PF has the significant protective effect against the H2O2-induced cell injury,which may be related to eliminatinging ROS,alleviating DNA oxidative damage,regulation of cell cycle,and inhibition of apoptosis of caspase pathway activation.
Publication Year: 2013
Publication Date: 2013-01-01
Language: en
Type: article
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Cited By Count: 1
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