Title: Study on the characteristics of mutations on rpoB and katG genes of mycobacterium tuberculosis circulating in Jiangsu Province
Abstract:Objective To describe the genotype characteristics of mycobacterium tuberculosis and its association with the drug resistant phenotype.To explore the molecular mechanisms of drug-resistant,so as to pr...Objective To describe the genotype characteristics of mycobacterium tuberculosis and its association with the drug resistant phenotype.To explore the molecular mechanisms of drug-resistant,so as to provide evidences for the rapid diagnosis and screening of drug-resistant mycobacterium tuberculosis.Methods The mutations of rifampin resistance related rpoB gene and isoniazid resistance related katG gene were amplified and sequenced.The association between genotypes and phenotypes of drug resistance were analysed.Results 255 mycobacterium tuberculosis strains were sequenced and 28 isolates(11.0%) were found having point mutations at the 81bp core area of rpoB gene.The main mutation was rpoB531 TCG→TTG(46.4%).Besides,two simultaneous point mutations were found at rpoB gene in seven strains.For katG gene,11.8% strains had mutations.All mutations were located at katG 315 position,with the mutation form of katG 315AGC→ACC.rpoB gene mutations all occurred in the rifampicin resistant strains and katG gene mutations all occurred in the isoniazid resistant strains with high sensitivity and specificity of 100% for both.Conclusions rpoB and katG gene mutations are important molecular mechanisms of mycobacterium tuberculosis to rifampicin and isoniazid resistance.Molecular epidemiological studies of mycobacterium tuberculosis are useful for understanding the mechanisms of drug resistance,and can help develop tools for early diagnosis of drug-resistant tuberculosis.Read More
Publication Year: 2013
Publication Date: 2013-01-01
Language: en
Type: article
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