Title: Rapid detection of gene mutation in multiple-drug resistant mycobacterium tuberculosis by PCR and single-strand conformation polymorphism analysis
Abstract:Objective To establish PCR-SSCP for rapid analyzing resistant gene in multiple drug resistance of mycobacterium tuberculosis and to evaluate its use in clinics. Method 30 mycobacterium tuberculosis st...Objective To establish PCR-SSCP for rapid analyzing resistant gene in multiple drug resistance of mycobacterium tuberculosis and to evaluate its use in clinics. Method 30 mycobacterium tuberculosis strains in multiple drug resistance were analyzed by PCR-SSCP and classic drug susceptibility tests. The fragments of rpoB, rpsL and katG were analyzed by single-stranded conformation polymorphism (SSCP). Results The gene mutation rate of rpoB, rpsL and katG resistance of isolated strains were 90% (27/30)?53% (16/30)?63%(19/30) Respectively. There are total 8(26.7%) strains with three genes mutation and 18(60%) strains with two genes mutation. Among the MDR-TB strains,there are 2 strains with single gene mutation and 2 strains without gene mutation. Conclusion Alterations in rpoB, rpsL, katG gene may be the important mechanisms of mycobacterium tuberculosis resistance to rifampin, streptomycin and isoniazid. The PCR-SSCP is an accurate and stable method to identify rpoB rpsL, katG showed mutation of mycobacterium tuberculosis, and it is useful for quickly identifying the MDR-TB strains in clinics.Read More
Publication Year: 2006
Publication Date: 2006-01-01
Language: en
Type: article
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