Title: Detection of the isoniazid-resistant katG gene of Mycobacterium tuberculosis.
Abstract:Objective To study the molecular mechanism of Mycobacterium tuberculosis resistance to isoniazid (INH) and to establish a method of rapid detection of katG gene mutations in M.tuberculosis.Methods The...Objective To study the molecular mechanism of Mycobacterium tuberculosis resistance to isoniazid (INH) and to establish a method of rapid detection of katG gene mutations in M.tuberculosis.Methods The katG genes of 105 M.tuberculosis clinical isolates were tested by PCR-RFLP and verified by DNA sequencing and bioinformatics analysis.Results Strain H37Rv was used as a control.In 105 M.tuberculosis clinical isolates,5 of 34 (14.7%) drug-susceptible isolates and 55 of 105 (52.4%) INH-resistant isolates had a katG gene mutation according to PCR-RFLP.Conclusion Drug resistance of M.tuberculosis to INH is related to katG gene mutation,PCR-RFLP might serve as a method of rapidly detecting the INH resistance of M.tuberculosis.Read More
Publication Year: 2010
Publication Date: 2010-01-01
Language: en
Type: article
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