Title: Effect of Arsenic Trioxide on Leukemia Cell Line HL-60
Abstract:Objective:To explore the effect of arsenic trioxide on cell apoptosis in HL-60 cells.Methods:DNA(deoxyribonucleic acid) line was detected by gelose electrophresis.Changes of cell nucleus stained by Ho...Objective:To explore the effect of arsenic trioxide on cell apoptosis in HL-60 cells.Methods:DNA(deoxyribonucleic acid) line was detected by gelose electrophresis.Changes of cell nucleus stained by Hoecst 33258 in HL-60 cells treated by arsenic trioxide were studied by fluorescence microscope.The apoptosis were detected by flow cytometry.Results:DNA fragment appeared after HL-60 cells exposed to 10 μmol/L arsenic trioxide from 24 to 48 h;cell nucleus were concentrated and fragmented after HL-60 cells exposed to 10 μmol/L arsenic trioxide for 48 h.Treated with 10 μmol/L arsenic trioxide for 12hours,24 hours and 48 h,the apoptotic rate was (8.5±2.1)%,(12.8±3.4)% and(21.4±5.8)%,respectively,but the apoptotic rate in the negative control group only (1.5±0.5)%(P0.05).Conclusion:Arsenic trioxide can induce apoptosis in HL-60 cells.Read More
Publication Year: 2009
Publication Date: 2009-01-01
Language: en
Type: article
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