Title: Effect of arsenic trioxide on leukemia cell line HL-60 differentiation
Abstract:OBJECTIVE: To explore the mechanism of arsenic trioxide(As2O3)in the treatment of leukemia. METHODS: 1)The expression of CD11b, cell cycle and apoptosis were investigated by flow cytometry. 2)The mRNA...OBJECTIVE: To explore the mechanism of arsenic trioxide(As2O3)in the treatment of leukemia. METHODS: 1)The expression of CD11b, cell cycle and apoptosis were investigated by flow cytometry. 2)The mRNA expression of c-myc was showed by RT-PCR. RESULTS: Arsenic trioxide significantly increased CD11b in HL-60 cells, and the expression rate of CD11b increased from (14.5±2.1)% to (35.4±5.7)% after treated by 2.5 μmol/L arsenic trioxide for 90 hours (P0.05), and the percentage of NBT positive cells increased from (10.0±2.1)% to (31.2±3.4)%(P0.05),and mRNA expression of c-myc was reduced significantly. CONCLUSION: Arsenic trioxide increases the cell differentiation in HL-60 cells by reducing mRNA expression of c-myc and increasing the expression of CD11b.Read More
Publication Year: 2008
Publication Date: 2008-01-01
Language: en
Type: article
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