Title: The biological function of miR-214 in colon cancer HCT116 cells
Abstract: Objective:To investigate the expression of miR-214(microRNA-214) in different colon cancer cell lines and its effect on the proliferation and apoptosis of HCT116 cells.Methods:The expressions of miR-214 and PIM1 mRNA in different colon cancer cell lines were detected by real-time fluorogenic quantitative-PCR,and the relationship between miR-214 and PIM1 mRNA expression was evaluated.The miR-214 mimics was transfected into HCT116 cells by LipofectAMINE 2000,then the expressions of miR-214 and PIM1 mRNA levels were detected by real-time fluorogenic quantitative-PCR.The change of proliferation ablility of HCT116 cells was detected by MTT method and colony-formation assay.The flow cytometry was used to examine the changes of apoptosis and cell cycle distribution.Results:The expression of miR-214 was down-regulated in HCT116 cells.The expression of miR-214 was negatively correlated with the expression of PIM1 mRNA in colon cancer cells(r=–0.943,P=0.016).After transfection with miR-214 mimics,the expression level of miR-214 was up-regulated as compared with no transfection.The high expression level of miR-214 increased in miR-214 mimics group compared with that in the negative control group(P0.01).The high expression level of miR-214 could significantly inhibit the expression of PIM1 mRNA.After transfection with miR-214 mimics,the number of colonies was decreased(P=0.026 9),the cell growth was inhibited(P0.000 1),the apoptosis rate was increased(P = 0.010 4),and the proportion of G1-stage cells was decreased as well as the proportion of S-stage cells was increased.Conclusion:MiR-214 can inhibit the proliferation and promote the apoptosis of colon cancer HCT116 cells.MiR-214 may act as a tumor suppressor in colorectal cancer by inhibiting PIM1.
Publication Year: 2013
Publication Date: 2013-01-01
Language: en
Type: article
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